MSp500- malp Cas9
(Plasmid
#227704)
-
PurposeCas9 under maltose promoter, natmx cassette
-
Depositing Lab
-
Depositing OrganizationNational Human Genome Research Institute (NHGRI)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepRS414-TEF1p-Cas9-CYC1t
- Total vector size (bp) 14179
-
Modifications to backbonereplaced tef promoter with. mal promoter
-
Vector typeYeast Expression, CRISPR
-
Selectable markerscloNAT
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCas9
- Promoter MAL
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CAGCCAACGTTATTTCTCAGGTCC
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSp500- malp Cas9 was a gift from Meru Sadhu (Addgene plasmid # 227704 ; http://n2t.net/addgene:227704 ; RRID:Addgene_227704)