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p-miR-Report + WT miR-181 BS in GATA6 3UTR
(Plasmid #22794)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22794 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22794-D50 50 μg of DNA in Tris buffer $495
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DNA 22794-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p-miR-Report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region
  • Alt name
    WT miR-181 BS in GATA6 3UTR
  • Alt name
    WT GATA6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    29
  • Entrez Gene
    GATA6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer AGGCGATTAAGTTGGGTA
  • 3′ sequencing primer ATTGCAACGATTTAGGTG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-miR-Report + WT miR-181 BS in GATA6 3UTR was a gift from Xin Wang (Addgene plasmid # 22794 ; http://n2t.net/addgene:22794 ; RRID:Addgene_22794)

  • For your References section:

    Identification of microRNA-181 by genome-wide screening as a critical player in EpCAM-positive hepatic cancer stem cells. Ji J, Yamashita T, Budhu A, Forgues M, Jia HL, Li C, Deng C, Wauthier E, Reid LM, Ye QH, Qin LX, Yang W, Wang HY, Tang ZY, Croce CM, Wang XW. Hepatology. 2009 Aug . 50(2):472-80. 10.1002/hep.22989 PubMed 19585654
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