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pMXs-IRES-blast 3xFlag-GFP-SLC45A1(E774A, L778A, L779A)
(Plasmid #228523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228523 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMXs-IRES-blast 3xFlag-GFP
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SLC45A1
  • Species
    H. sapiens (human)
  • Mutation
    At position 774, glutamic acid (E) is replaced by alanine (A), at position 778, leucine (L) is replaced by alanine (A), at position 779, leucine (L) is replaced by alanine (A).
  • GenBank ID
    NM_001080397.2
  • Entrez Gene
    SLC45A1 (a.k.a. DNB5, IDDNPF, PAST-A)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GCTTGGATACACGCCGC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Codon-optimized SLC45A1 orf was amplified from pDONR221_SLC45A1 plasmid (Addgene Plasmid #132289)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-IRES-blast 3xFlag-GFP-SLC45A1(E774A, L778A, L779A) was a gift from Monther Abu-Remaileh (Addgene plasmid # 228523 ; http://n2t.net/addgene:228523 ; RRID:Addgene_228523)

  • For your References section:

    Cell-type resolved protein atlas of brain lysosomes identifies SLC45A1-associated disease as a lysosomal disorder. Ghoochani A, Heiby JC, Rawat ES, Medoh UN, Di Fraia D, Dong W, Gastou M, Rastogi M, Hernandez V, Nyame K, Laqtom NN, Durso W, Valkova C, Isakova A, Kaether C, Wernig M, Gomez-Ospina N, Franke C, Ori A, Abu-Remaileh M. Cell. 2026 Feb 5;189(3):765-782.e31. doi: 10.1016/j.cell.2025.12.012. Epub 2026 Jan 22. 10.1016/j.cell.2025.12.012 PubMed 41576950
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