pR-mTagBFP2
(Plasmid
#228596)
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PurposeMammalian expression of mTagBFP2, for MuSIC barcode construction
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Depositing Lab
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Depositing OrganizationClemson University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 228596-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 228596-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepReceiver
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Backbone manufacturerBirtwistle lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2
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SpeciesSynthetic
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Insert Size (bp)711
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MutationWe introduced some silent mutations to avoid essential restriction enzyme sites.
- Promoter CMV
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.10.23.619855 for bioRxiv preprint.
DNA (Catalog # 228596-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 228596-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pR-mTagBFP2 was a gift from Marc Birtwistle (Addgene plasmid # 228596 ; http://n2t.net/addgene:228596 ; RRID:Addgene_228596) -
For your References section:
Genetically Encoded Fluorescence Barcodes Allow for Single-Cell Analysis via Spectral Flow Cytometry. Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR. ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 10.1021/acssynbio.4c00807. Epub 2025 May 6. 10.1021/acssynbio.4c00807 PubMed 40326708
