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mCherry_EHMT1_full length
(Plasmid #228810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228810 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 228810-D50 50 μg of DNA in Tris buffer $495
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DNA 228810-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmCherry_C1
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EHMT1
  • Alt name
    G9a-like protein (GLP)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3894
  • Mutation
    See Depositor Comments
  • Entrez Gene
    Ehmt1 (a.k.a. 9230102N17Rik, D330003E03, Eu-HMTase1, GLP, GLP1, KMT1D, mKIAA1876)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Subcloned from V5-EHMT1 plasmid (doi: 10.15252/embr.201643260)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E240R mutation in the EHMT1 insert should not affect basal functionality.

Please visit https://doi.org/10.1101/2023.11.01.564969 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry_EHMT1_full length was a gift from Shravanti Rampalli (Addgene plasmid # 228810 ; http://n2t.net/addgene:228810 ; RRID:Addgene_228810)

  • For your References section:

    A cytoplasmic form of EHMT1N methylates viral proteins to enable inclusion body maturation and efficient viral replication. Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S. PLoS Biol. 2024 Nov 7;22(11):e3002871. doi: 10.1371/journal.pbio.3002871. eCollection 2024 Nov. 10.1371/journal.pbio.3002871 PubMed 39509467
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