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pRSVBneoT
(Plasmid #22883)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22883 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM3zf(+)
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Early region SV40
  • Species
    SV40
  • Insert Size (bp)
    2658

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid was made more than 20 years ago. The vector (pGEM3zf(+)) originated from Promega while the cassette (pMCneo(PolyA)) from Stratagene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSVBneoT was a gift from James Pipas (Addgene plasmid # 22883)
  • For your References section:

    The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain. Srinivasan A, McClellan AJ, Vartikar J, Marks I, Cantalupo P, Li Y, Whyte P, Rundell K, Brodsky JL, Pipas JM. Mol Cell Biol. 1997 Aug . 17(8):4761-73. PubMed 9234732