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peGFP-Chk1
(Plasmid #22888)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22888 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chk1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1430
  • GenBank ID
    CHEK1
  • Entrez Gene
    CHEK1 (a.k.a. CHK1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The following residue changes were found during quality control: D130A and L257P. These mutations are of no known functional significance.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peGFP-Chk1 was a gift from Aziz Sancar (Addgene plasmid # 22888)
  • For your References section:

    Coupling of human circadian and cell cycles by the timeless protein. Unsal-Kacmaz K, Mullen TE, Kaufmann WK, Sancar A. Mol Cell Biol. 2005 Apr . 25(8):3109-16. 10.1128/MCB.25.8.3109-3116.2005 PubMed 15798197