pCDNA5-LRRK2
(Plasmid #229019)

• Purpose: Expression of untagged full length human LRRK2 in mammalian cells
• Depositing Lab: Samara Reck-Peterson
• Depositing Organization: University of California, San Diego (UCSD)
  Located in United States of America
• Publication: Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi: 10.1016/j.jbc.2024.107469. Epub 2024 Jun 12.
• Citations: 1 Article

Backbone

• Vector backbone: pCDNA5
• Backbone manufacturer: Genescript
• Backbone size w/o insert (bp): 5094
• Total vector size (bp): 12678
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Leucine-rich repeat kinase 2
• Alt name: LRRK2
• Species: H. sapiens (human)
• Insert Size (bp): 7584
• Mutation: none
• GenBank ID: NM_198578.4 NM_198578.4
• Entrez Gene: LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
• Promoter: CMV
• Tag / Fusion Protein:
  • no tags (untagged)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: Plasmidsaurus full sequencing

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GeneScript. We designed and ordered it as synthesized plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also used in : Type-II kinase inhibitors that target Parkinson’s Disease-associated LRRK2
Nicolai D. Raig, Katherine J. Surridge, Marta Sanz-Murillo, Verena Dederer, Andreas Krämer, Martin P. Schwalm, Lewis Elson, Deep Chatterjee, Sebastian Mathea, Thomas Hanke, Andres E. Leschziner, Samara L. Reck-Peterson, Stefan Knapp
bioRxiv 2024.09.17.613365; doi: https://doi.org/10.1101/2024.09.17.613365

DNA (Catalog # 229019-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 229019-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.