|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22917||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namefugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP
Speciestakifugu rubripes (fugu)
Insert Size (bp)4300
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Depositor used a previous fugu genome sequence, and this may be different to the
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-fCAMK-GFP was a gift from Edward Callaway (Addgene plasmid # 22917 ; http://n2t.net/addgene:22917 ; RRID:Addgene_22917)
For your References section:Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461