pAAV-DR1-NRSE-RFP
(Plasmid #22927)

• Depositing Lab: Edward Callaway
• Publication: Nathanson et al Front Neural Circuits. 2009 . 3():19.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
• Alt name: Pparg DR1
• Insert Size (bp): 1700

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a gap in alignment between depositor's sequence
and Addgene's sequence. The gap is probably part of the cloning site. Also, this promoter sequence
is largely artificial, and won't show by BLAST.