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pcDNA FLAG TORC1 (1-630)
(Plasmid #22974)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22974 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TORC1 (1-630)
  • Alt name
    TORC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    Amino acids 1-630 rather than 1-634.
  • Entrez Gene
    CRTC1 (a.k.a. MAML2, MECT1, Mam-2, TORC-1, TORC1, WAMTP1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

There is an XbaI site at the 3' end instead of a NotI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA FLAG TORC1 (1-630) was a gift from Marc Montminy (Addgene plasmid # 22974 ; http://n2t.net/addgene:22974 ; RRID:Addgene_22974)
  • For your References section:

    TORCs: transducers of regulated CREB activity. Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M. Mol Cell. 2003 Aug . 12(2):413-23. 10.1016/j.molcel.2003.08.013 PubMed 14536081