pMX231
(Plasmid
#23003)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4731
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKif18A-mut
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Alt nameKif18A
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SpeciesH. sapiens (human)
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Insert Size (bp)2731
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MutationH304A R308A K311A
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GenBank IDBC048347
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Entrez GeneKIF18A (a.k.a. MS-KIF18A, PPP1R99)
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Tags
/ Fusion Proteins
- EGFP (N terminal on backbone)
- 6His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX231 was a gift from Linda Wordeman (Addgene plasmid # 23003 ; http://n2t.net/addgene:23003 ; RRID:Addgene_23003) -
For your References section:
The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment. Stumpff J, von Dassow G, Wagenbach M, Asbury C, Wordeman L. Dev Cell. 2008 Feb . 14(2):252-62. 10.1016/j.devcel.2007.11.014 PubMed 18267093
