pMX234
(Plasmid #23006)

• Depositing Lab: Linda Wordeman
• Publication: Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Backbone

• Vector backbone: pCMV-M1
• Backbone size w/o insert (bp): 4004
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRFP-CenpB
• Alt name: CENP-B
• Alt name: centromere autoantigen protein B
• Species: Cricetulus griseus
• Insert Size (bp): 1214
• Mutation: deleted CenpB amino acids 169-606, T145S
• GenBank ID: U20951
• Tag / Fusion Protein:
  • mRFP1.0 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV promoter

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry