pMX241
(Plasmid
#23017)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 23017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGFPCPB1
- Backbone size w/o insert (bp) 5205
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCPBMhyp
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Alt nameMCAK
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SpeciesCricetulus griseus
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Insert Size (bp)1227
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Mutationdeleted MCAK amino acids 1-178, 584-718 mutated S186A, H530A, R534A, K537A
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GenBank IDU11790
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Tags
/ Fusion Proteins
- EGFP (N terminal on backbone)
- CenpB 1-167 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX241 was a gift from Linda Wordeman (Addgene plasmid # 23017 ; http://n2t.net/addgene:23017 ; RRID:Addgene_23017) -
For your References section:
MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover. Wordeman L, Wagenbach M, von Dassow G. J Cell Biol. 2007 Dec 3. 179(5):869-79. 10.1083/jcb.200707120 PubMed 18039936
