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xPERT-hA2q
(Plasmid #231063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231063 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 231063-D50 50 μg of DNA in Tris buffer $495
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DNA 231063-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pC0053
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 9642
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    dCas13d
  • Species
    H. sapiens (human); Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    3060
  • Mutation
    K490L
  • Promoter CMV

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Human deaminase ADAR2dd
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    1154
  • Mutation
    H460D, E488Q, only the deaminase domain (aa 276-702 are expressed)
  • Entrez Gene
    ADARB1 (a.k.a. ADAR2, DRABA2, DRADA2, NEDHYMS, RED1)
  • Promoter CMV

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xPERT-hA2q was a gift from Meng How Tan (Addgene plasmid # 231063 ; http://n2t.net/addgene:231063 ; RRID:Addgene_231063)

  • For your References section:

    A circularly permuted CasRx platform for efficient, site-specific RNA editing. Wang Y, Liu KI, Liu MM, Ooi KH, Nguyen TA, Chee JE, Teo SXD, He S, Tay JWD, Teo SY, Liew KS, Ge XY, Ng ZJ, Avagyan H, Liu H, Yi Z, Chang K, Kok EPL, Chen R, Yau CE, Koh JW, Wan Y, Tan MH. Nat Biotechnol. 2024 Oct 9. doi: 10.1038/s41587-024-02430-w. 10.1038/s41587-024-02430-w PubMed 39385008
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