B23(9-122)
(Plasmid #23142)

• Depositing Lab: SeWon Suh
• Publication: Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

Backbone

• Vector backbone: pET21a(+)
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B23 core
• Alt name: Nucleophosmin core
• Species: H. sapiens (human)
• Insert Size (bp): 342
• Mutation: deleted amino acids 1-8
• GenBank ID: ABP97432
• Entrez Gene: NPM1 (a.k.a. B23, NPM)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry