pNH-TrxT-RPS2
(Plasmid
#231489)
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PurposeExpresses human ribosomal protein S2/uS5 with an N-terminal His-TrxA tag in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 231489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepNH-TrxT
- Backbone size w/o insert (bp) 7598
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRPS2
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Alt nameHuman ribosomal protein S2/uS5
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SpeciesH. sapiens (human)
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Entrez GeneRPS2 (a.k.a. LLREP3, S2, uS5)
- Promoter T7
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Tags
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- Thioredoxin A (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH-TrxT-RPS2 was a gift from Quira Zeidan (Addgene plasmid # 231489 ; http://n2t.net/addgene:231489 ; RRID:Addgene_231489) -
For your References section:
Cloning, expression, and purification of small subunit human ribosomal proteins SA/uS2, S2/uS5, S3/uS3, S7/eS7, S8/eS8, S12/eS12, S13/uS15, and S14/uS11 for in vitro characterization. Nicholl S, Thompson M, Goshtasbi-Gowharrizi A, Streit J, Martin J, Outmezguine D, Charkhabi A, Zeidan Q. MicroPubl Biol. 2026 Jul 9;2026:10.17912/micropub.biology.002010. doi: 10.17912/micropub.biology.002010. eCollection 2026. 10.17912/micropub.biology.002010 PubMed 42502757