AAVS1_Bxb1-GA_LP_Dual_TC
(Plasmid
#231943)
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PurposeAAVS1 targeting construct with the Bxb1-GA landing pad cassette.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 231943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBR322/pUC
- Total vector size (bp) 8161
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRT-mScarlet-attP(GA)-PuroR-F3
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SpeciesSynthetic
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Insert Size (bp)4200
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MutationN/A
- Promoter PGK
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1_Bxb1-GA_LP_Dual_TC was a gift from Richard Davis (Addgene plasmid # 231943 ; http://n2t.net/addgene:231943 ; RRID:Addgene_231943) -
For your References section:
Generation of a STRAIGHT-IN Dual AAVS1 hiPSC line with orthogonal landing pads for versatile DNA payload integration. van der Vaart B, Blanch-Asensio A, Geijsen N, Mummery CL, Freund C, Davis RP. Stem Cell Res. 2026 Jun 6;95:104028. doi: 10.1016/j.scr.2026.104028. 10.1016/j.scr.2026.104028 PubMed 42263534
