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p5953 pcDNA-Xpress-His-USP15
(Plasmid #23216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23216 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 23216-D50 50 μg of DNA in Tris buffer $495
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DNA 23216-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA-4C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP15
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2900
  • Entrez Gene
    USP15 (a.k.a. UNPH-2, UNPH4)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Xpress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5953 pcDNA-Xpress-His-USP15 was a gift from Peter Howley (Addgene plasmid # 23216 ; http://n2t.net/addgene:23216 ; RRID:Addgene_23216)

  • For your References section:

    The ubiquitin-specific peptidase USP15 regulates human papillomavirus type 16 E6 protein stability. Vos RM, Altreuter J, White EA, Howley PM. J Virol. 2009 Sep . 83(17):8885-92. 10.1128/JVI.00605-09 PubMed 19553310
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