Skip to main content
Addgene
Open Menu

pU6_empty-sgRNA(PP7)
(Plasmid #232432)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232432 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 232432-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 232432-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    phU6
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PP7-tagged sgRNA scaffold driven by human U6 promoter
  • gRNA/shRNA sequence
    empty (BbsI restriction sites)
  • Species
    Synthetic; Multiple
  • Promoter U6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HELMHOLTZ MUNICH has filed a patent (PCT/EP2025/053730) disclosing the capabilities of Material, and retains ownership rights to pU6_empty-sgRNA(PP7) Material.

Recipient agrees not to file for any intellectual property protection for Material.

Please note that any use of the Material or the HELMHOLTZ MUNICH Information for any commercial purpose - or by, on behalf of or in collaboration with any for-profit entity - requires a license from HELMHOLTZ MUNICH. To obtain such a license, please contact HELMHOLTZ MUNICH by directing your request to: [email protected]

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6_empty-sgRNA(PP7) was a gift from Gil Westmeyer (Addgene plasmid # 232432 ; http://n2t.net/addgene:232432 ; RRID:Addgene_232432)

  • For your References section:

    Engineered nucleocytosolic vehicles for loading of programmable editors. Geilenkeuser J, Armbrust N, Steinmassl E, Du SW, Schmidt S, Binder EMH, Li Y, Warsing NW, Wendel SV, von der Linde F, Schiele EM, Niu X, Stroppel L, Berezin O, Santl TH, Orschmann T, Nelson K, Gruber C, Palczewska G, Menezes CR, Risaliti E, Engfer ZJ, Koleci N, Schmidts A, Geerlof A, Palczewski K, Westmeyer GG, Truong DJ. Cell. 2025 May 15;188(10):2637-2655.e31. doi: 10.1016/j.cell.2025.03.015. Epub 2025 Apr 9. 10.1016/j.cell.2025.03.015 PubMed 40209705
Related items:
Commonly requested with: