pScreen-S1.3
(Plasmid
#232671)
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PurposeExpresses GFP in the presence of (S)-1-Phenyl-1,2,3,4-tetrahydroisoquinoline
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonep15A_KanR
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Backbone manufacturerTwist
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRamR-S1.3
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SpeciesSynthetic
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MutationC135E, S138D, V139N
Cloning Information
- Cloning method Gene Synthesis
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-5861295/v1 for Research Square preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pScreen-S1.3 was a gift from Simon d'Oelsnitz & Pamela Silver (Addgene plasmid # 232671 ; http://n2t.net/addgene:232671 ; RRID:Addgene_232671) -
For your References section:
Using enantioselective biosensors to evolve asymmetric biocatalysts. d’Oelsnitz S, Kim W, Hardtke H, Ikonomova SP, Alperovich N, Vasilyeva O, James MJ, Zigon ES, Johnson C, Ellington AD, Justman Q, Springer M, Zhang YJ, Silver PA, Ross D. Research Square [Preprint] 2025 Jan 30. doi: 10.21203/rs.3.rs-5861295/v1. 10.21203/rs.3.rs-5861295/v1