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pScreen-S1.3
(Plasmid #232671)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232671 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    p15A_KanR
  • Backbone manufacturer
    Twist
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RamR-S1.3
  • Species
    Synthetic
  • Mutation
    C135E, S138D, V139N

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-5861295/v1 for Research Square preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pScreen-S1.3 was a gift from Simon d'Oelsnitz & Pamela Silver (Addgene plasmid # 232671 ; http://n2t.net/addgene:232671 ; RRID:Addgene_232671)
  • For your References section:

    Using enantioselective biosensors to evolve asymmetric biocatalysts. d’Oelsnitz S, Kim W, Hardtke H, Ikonomova SP, Alperovich N, Vasilyeva O, James MJ, Zigon ES, Johnson C, Ellington AD, Justman Q, Springer M, Zhang YJ, Silver PA, Ross D. Research Square [Preprint] 2025 Jan 30. doi: 10.21203/rs.3.rs-5861295/v1. 10.21203/rs.3.rs-5861295/v1