pUC57-FlpTag-3xV5-ph1
(Plasmid #232874)

• Purpose: Encodes FlpTag with 3xV5 epitope tag in phase 1. An inverted tag enables conditional tagging and visualization of endogenous proteins upon Flp recombinase activity.
• Depositing Lab: Lukas Groschner
• Depositing Organization: Max Planck Institute for Biological Intelligence
  Located in Germany
• Publication: Vieira et al Proc Natl Acad Sci U S A. 2026 Oct 6;123(40):e2610600123. doi: 10.1073/pnas.2610600123. Epub 2026 Sep 29.

Backbone

• Vector backbone: pUC57-FlpTag-GFP-ph0
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Inverted 3xV5
• Species: Synthetic
• Insert Size (bp): 688
• Tag / Fusion Protein:
  • V5 epitope tag

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: caggaaacagctatgacc
• 3′ sequencing primer: tgtaaaacgacggccagt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The FlpTag insertion consists of an inverted sequence containing a splice acceptor (SA), 3xV5, and a splice donor (SD), flanked on either side by FRT and FRT14 recombination sites. This configuration allows conditional protein tagging, where the cassette orientation is controlled by Flp recombinase, enabling specific expression in targeted cells or tissues. FlpTag is integrated into the genome at specific attP sites, targeting the gene of interest through phiC31 integrase-mediated recombination at the designated gene locus.