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Reckleen_2_SpGCas9
(Plasmid #233457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233457 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 233457-D50 50 μg of DNA in Tris buffer $495
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DNA 233457-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Custom built using Marburg Collection
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Level 2 RECKLEEN plasmid
  • Alt name
    Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4, Ptet sfgfp dropout sgRNA
  • Alt name
    pMB1 ori, and ApmR (aac(3)-IV)
  • Species
    Other

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-5859838/v1 for preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Reckleen_2_SpGCas9 was a gift from Anke Becker (Addgene plasmid # 233457 ; http://n2t.net/addgene:233457 ; RRID:Addgene_233457)

  • For your References section:

    RECKLEEN is a lambda Red/CRISPR-Cas9 based single plasmid platform for enhanced genome editing in Klebsiella pneumoniae. Elsayed EM, Stukenberg D, Meier D, Schmeck B, Becker A. Commun Biol. 2025 Oct 30;8(1):1509. doi: 10.1038/s42003-025-08934-8. 10.1038/s42003-025-08934-8 PubMed 41168338
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