psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL
(Plasmid
#233628)
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PurposeExpression of bicistronic reporter with TOP motif
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Depositing Lab
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Depositing OrganizationRIKEN Pioneering Research Institute, Wako
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 233628-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 233628-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepsiCHECK2
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePromoter and 5'UTR of Eef2 gene and HCV IRES
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SpeciesM. musculus (mouse)
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Entrez GeneEef2 (a.k.a. Ef-2)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 233628-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 233628-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL was a gift from Shintaro Iwasaki (Addgene plasmid # 233628 ; http://n2t.net/addgene:233628 ; RRID:Addgene_233628) -
For your References section:
eIF4A1 enhances LARP1-mediated translational repression during mTORC1 inhibition. Shichino Y, Yamaguchi T, Kashiwagi K, Mito M, Takahashi M, Ito T, Ingolia NT, Kuba K, Iwasaki S. Nat Struct Mol Biol. 2024 Oct;31(10):1557-1566. doi: 10.1038/s41594-024-01321-7. Epub 2024 May 21. 10.1038/s41594-024-01321-7 PubMed 38773334
