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pBiEx-1 MyLID-IIB-N
(Plasmid #233730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233730 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBiEx-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5263
  • Total vector size (bp) 10342
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MyLID-IIB-N
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    5079
  • GenBank ID
    NM_001256012
  • Promoter IE1
  • Tags / Fusion Proteins
    • FLAG-tag (N terminal on insert)
    • HaloTag (N terminal on insert)
    • LOV2-SsrA (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer ccgcggaaattaatacga
  • 3′ sequencing primer GAGGTTAATCACTTAGTGCT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.19.666446 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiEx-1 MyLID-IIB-N was a gift from Zev Bryant (Addgene plasmid # 233730 ; http://n2t.net/addgene:233730 ; RRID:Addgene_233730)

  • For your References section:

    Engineering filamentous myosins for optical control of contractility. Zemsky S, Ruijgrok PV, Bryant Z. bioRxiv 2025.08.19.666446 10.1101/2025.08.19.666446
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