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pMT MyLOV-XI-IIB
(Plasmid #233732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233732 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMT-V5/His B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3403
  • Total vector size (bp) 11914
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MyLOV-XI-IIB
  • Alt name
    myosin-6
  • Species
    H. sapiens (human), Synthetic; Nicotiana tabacum
  • Insert Size (bp)
    8511
  • GenBank ID
    NM_001256012 AB082121
  • Entrez Gene
    MYH10 (a.k.a. NMMHC-IIB, NMMHCB)
  • Entrez Gene
    MYO6 (a.k.a. DFNA22, DFNB37)
  • Promoter Metallothionein
  • Tag / Fusion Protein
    • mRuby3 (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.19.666446 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT MyLOV-XI-IIB was a gift from Zev Bryant (Addgene plasmid # 233732 ; http://n2t.net/addgene:233732 ; RRID:Addgene_233732)

  • For your References section:

    Engineering filamentous myosins for optical control of contractility. Zemsky S, Ruijgrok PV, Bryant Z. bioRxiv 2025.08.19.666446 10.1101/2025.08.19.666446
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