RMCE ABCDE>ala DNA-PKcs
(Plasmid
#233914)
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PurposeLow copy number provides expression of human DNA-PKcs with the 6 ABCDE phosphorylation sites substituted to alanine.
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Depositing Lab
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Depositing OrganizationMichigan State University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233914 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 233914-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 233914-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepsm7
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman DNA-PKcs ABCDE phosphorylation site mutant
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SpeciesH. sapiens (human)
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Mutationphosphorylation sites 2609, 2612, 2620, 2624, 2638, 2647 alanine substituted
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Entrez GenePRKDC (a.k.a. DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 233914-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 233914-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RMCE ABCDE>ala DNA-PKcs was a gift from Katheryn Meek (Addgene plasmid # 233914 ; http://n2t.net/addgene:233914 ; RRID:Addgene_233914) -
For your References section:
Two distinct long-range synaptic complexes promote different aspects of end processing prior to repair of DNA breaks by non-homologous end joining. Buehl CJ, Goff NJ, Hardwick SW, Gellert M, Blundell TL, Yang W, Chaplin AK, Meek K. Mol Cell. 2023 Mar 2;83(5):698-714.e4. doi: 10.1016/j.molcel.2023.01.012. Epub 2023 Jan 31. 10.1016/j.molcel.2023.01.012 PubMed 36724784
