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makeTCR_Hs.TRBV9
(Plasmid #234037)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234037 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pmakeTCR
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 1710
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRBV9
  • Alt name
    ENSG00000261031
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    333
  • Entrez Gene
    TRBV9 (a.k.a. TCRBV1S1A1N1, TCRBV9S1)
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site CCAT (not destroyed)
  • 3′ cloning site TGTG (not destroyed)
  • 5′ sequencing primer M13 (-21) Forward
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.04.27.647198 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    makeTCR_Hs.TRBV9 was a gift from Michael Platten (Addgene plasmid # 234037 ; http://n2t.net/addgene:234037 ; RRID:Addgene_234037)

  • For your References section:

    makeTCR: A Modular Platform for Rapid, Flexible, Scalable, Single-Step T Cell Receptor Synthesis. Hamberger M, Neuhoff M-T, Pietrantonio SV, Boschert T, Maldonado Torres C, Errerd A, Tan CL, Lindner JM, Platten M, Green EW. bioRxiv 2025.04.27.647198 10.1101/2025.04.27.647198
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