pOPINHuVH
(Plasmid
#234057)
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PurposeExpression of human antibody Fd heavy chains - contains huIgG1 CH 1 sequence
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepOPING
- Backbone size w/o insert (bp) 5594
- Total vector size (bp) 5906
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Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman CH1 heavy chain fragment (Fd)
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Alt nameheavy chain
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINHuVH was a gift from Ray Owens (Addgene plasmid # 234057 ; http://n2t.net/addgene:234057 ; RRID:Addgene_234057) -
For your References section:
Structural and functional mapping of protective human monoclonal antibodies against enterovirus A71. Zhou D, Kotecha A, Kelly JT, Huang PN, Chen YY, Walter TS, Duyvesteyn HME, Owens RJ, Ho SY, Lin TY, Fry EE, Ren J, Huang KA, Stuart DI. Sci Adv. 2026 Jun 5;12(23):eaee8217. doi: 10.1126/sciadv.aee8217. Epub 2026 Jun 5. 10.1126/sciadv.aee8217 PubMed 42247493
Map uploaded by the depositor.
