FLARE-miniV10-10x
(Plasmid
#234492)
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PurposeExpresses the FLARE lineage tracing recorder, contains a UMI insertion site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234492 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePB-CMV-MCS-EF1-puro
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Vector typeMammalian Expression
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLARE lineage recorder target array
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SpeciesSynthetic
- Promoter Ef1alpha
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Tag
/ Fusion Protein
- EGFP
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TCGGCATGGACGAGCTGTACAAGT
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.21.586160 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLARE-miniV10-10x was a gift from Aaron McKenna (Addgene plasmid # 234492 ; http://n2t.net/addgene:234492 ; RRID:Addgene_234492) -
For your References section:
Hierarchical lineage tracing reveals diverse pathways of cytarabine resistance. Saxe R, Stuart H, Marshall AC, Abdullahi F, Chen Z, Emiliani F, McKenna A. Nat Commun. 2026 Jun 30. doi: 10.1038/s41467-026-74989-8. 10.1038/s41467-026-74989-8 PubMed 42380134