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FLARE-pbV10g-neo
(Plasmid #234493)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234493 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PB-CMV-MCS-EF1-puro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLARE guide RNAs
  • gRNA/shRNA sequence
    FLARE lineage recorder targets
  • Species
    Synthetic
  • Promoter U6

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.21.586160 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLARE-pbV10g-neo was a gift from Aaron McKenna (Addgene plasmid # 234493 ; http://n2t.net/addgene:234493 ; RRID:Addgene_234493)

  • For your References section:

    Hierarchical lineage tracing reveals diverse pathways of cytarabine resistance. Saxe R, Stuart H, Marshall AC, Abdullahi F, Chen Z, Emiliani F, McKenna A. Nat Commun. 2026 Jun 30. doi: 10.1038/s41467-026-74989-8. 10.1038/s41467-026-74989-8 PubMed 42380134
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