pSL1180-NiV L(2k)-mCherry/eGFP
(Plasmid
#234654)
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PurposeMinigenome plasmid of NiV Bangladesh strain
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Depositing Lab
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Depositing OrganizationWashington University in Saint Louis
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 234654-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 234654-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSL1180
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Vector typeMammalian Expression ; in cell lines expressing bacteriophage T7 RNA polymerase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namepart L gene
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SpeciesNipah virus
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Insert Size (bp)2002
Gene/Insert 2
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Gene/Insert namemCherry
Gene/Insert 3
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Gene/Insert nameeGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 234654-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 234654-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSL1180-NiV L(2k)-mCherry/eGFP was a gift from Carolina Lopez (Addgene plasmid # 234654 ; http://n2t.net/addgene:234654 ; RRID:Addgene_234654) -
For your References section:
Indiscriminate activities of different henipavirus polymerase complex proteins allow for efficient minigenome replication in hybrid systems. Li X, Yang Y, Lopez CB. J Virol. 2024 Jun 13;98(6):e0050324. doi: 10.1128/jvi.00503-24. Epub 2024 May 23. 10.1128/jvi.00503-24 PubMed 38780245
Map uploaded by the depositor.
