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pcDNA3.1-ENE-YFP
(Plasmid #234935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234935 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDH1
  • Species
    H. sapiens (human)
  • Mutation
    E-cad a.a: 523 - 594 is replaced by N-cad a.a 535 - 603
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII, NheI (not destroyed)
  • 3′ cloning site XbaI, ApaI (not destroyed)
  • 5′ sequencing primer CMV primer
  • 3′ sequencing primer EGFP primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We originally received this clone from Keith R Johnson and Margaret J Wheelock.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-ENE-YFP was a gift from Deborah Leckband (Addgene plasmid # 234935 ; http://n2t.net/addgene:234935 ; RRID:Addgene_234935)

  • For your References section:

    Epidermal growth factor receptor is an essential component in E-cadherin force transduction complexes. Zou Y, Allen N, Rauf E, Leckband D. J Cell Sci. 2025 Nov 1;138(21):jcs264350. doi: 10.1242/jcs.264350. Epub 2025 Nov 13. 10.1242/jcs.264350 PubMed 40908853
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