pHA TST 3C SMO EABR
(Plasmid
#234989)
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PurposeFor production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface
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Depositing Labs
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Depositing OrganizationUniversity of Texas at Austin
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 234989-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 234989-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSMO-EABR
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Alt nameSmoothened (Smo) fused to ESCRT and Alix binding region (EABR)
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SpeciesH. sapiens (human)
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GenBank IDPV031345
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Entrez GeneSMO (a.k.a. CRJS, FZD11, Gx, PHLS, SMOH)
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Tag
/ Fusion Protein
- HA, Twin-Strep-Tag, HRV 3C site (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains an A25V mutation in NeoR/KanR. This mutation is not expected to impact plasmid function.
DNA (Catalog # 234989-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 234989-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHA TST 3C SMO EABR was a gift from Monica Gonzalez-Magaldi & Daniel Leahy (Addgene plasmid # 234989 ; http://n2t.net/addgene:234989 ; RRID:Addgene_234989) -
For your References section:
Structure and organization of full-length Epidermal Growth Factor Receptor in extracellular vesicles by cryo-electron tomography. Gonzalez-Magaldi M, Gullapalli A, Papoulas O, Liu C, Leung AY, Guo L, Brilot A, Marcotte EM, Ke Z, Leahy DJ. bioRxiv [Preprint]. 2024 Nov 28:2024.11.25.625301. doi: 10.1101/2024.11.25.625301. 10.1101/2024.11.25.625301 PubMed 39651119
