pCAG-MCP-dNS-SRRM1-V5-mCherry
(Plasmid
#235095)
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PurposeSRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain
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Depositing Lab
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Depositing OrganizationCalifornia Institute of Technology
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235095 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 235095-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 235095-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAG-NSTF-DEST-V5-mCherry
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCAG-MCP-dNS-SRRM1-V5-mCherry
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SpeciesH. sapiens (human)
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MutationThe SRRM1 entry clone from DNASU was modified using a Q5 site directed mutagenesis kit (New England Biolabs) to delete the predicted region responsible for nuclear speckle localization as annotated using UniProt (https://www.uniprot.org/uniprotkb/Q8IYB3/entry)
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Entrez GeneSRRM1 (a.k.a. 160-KD, POP101, SRM160)
- Promoter CAG
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 235095-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 235095-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-MCP-dNS-SRRM1-V5-mCherry was a gift from Mitchell Guttman (Addgene plasmid # 235095 ; http://n2t.net/addgene:235095 ; RRID:Addgene_235095) -
For your References section:
Genome organization around nuclear speckles drives mRNA splicing efficiency. Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh ST, Chari T, Sullivan DK, Blanco MR, Guttman M. Nature. 2024 May;629(8014):1165-1173. doi: 10.1038/s41586-024-07429-6. Epub 2024 May 8. 10.1038/s41586-024-07429-6 PubMed 38720076
