pB–DualPE
(Plasmid
#235161)
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PurposeFor plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons
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Depositing Lab
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Depositing OrganizationChina Agricultural University
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 235161-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 235161-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBUE411
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Vector typeCRISPR
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCsy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
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SpeciesSynthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
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Insert Size (bp)6807
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MutationDetailed in manuscript
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer Ubi-F
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCmYLCV-epegRNA-CaMV poly(A) signal
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SpeciesSynthetic
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Insert Size (bp)841
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MutationDetailed in manuscript
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer M13F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 235161-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 235161-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB–DualPE was a gift from Zong Yuan (Addgene plasmid # 235161 ; http://n2t.net/addgene:235161 ; RRID:Addgene_235161) -
For your References section:
Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing. Zhao Y, Huang Z, Zhou X, Teng W, Liu Z, Wang W, Tang S, Liu Y, Liu J, Wang W, Chai L, Zhang N, Guo W, Liu J, Ni Z, Sun Q, Wang Y, Zong Y. Nat Plants. 2025 Feb;11(2):191-205. doi: 10.1038/s41477-024-01898-3. Epub 2025 Jan 13. 10.1038/s41477-024-01898-3 PubMed 39805934
Map uploaded by the depositor.
