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Redi.1.AsCas12a-L218S-I285S-L972A
(Plasmid #235234)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235234 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5354
  • Total vector size (bp) 9464
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hAsCpf1
  • Alt name
    AsCas12a
  • Species
    Acidaminococcus_sp_BV3L6
  • Insert Size (bp)
    3921
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Redi.1.AsCas12a-L218S-I285S-L972A was a gift from Feng Zhang (Addgene plasmid # 235234 ; http://n2t.net/addgene:235234 ; RRID:Addgene_235234)

  • For your References section:

    Rational engineering of minimally immunogenic nucleases for gene therapy. Raghavan R, Friedrich MJ, King I, Chau-Duy-Tam Vo S, Strebinger D, Lash B, Kilian M, Platten M, Macrae RK, Song Y, Nivon L, Zhang F. Nat Commun. 2025 Jan 2;16(1):105. doi: 10.1038/s41467-024-55522-1. 10.1038/s41467-024-55522-1 PubMed 39747875
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