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STK080_pSTK-0D-bkdB Cold double terminator
(Plasmid #235414)

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  • Purpose
    Very strong terminator in B. subtilis. Transcriptional terminator and 3'-mRNA stabilizer from bkdB gene of B. subtilis. Positive impact in expression at 18C. Contain STOP codons in both directions
  • Depositing Lab
    Tom Ellis
  • Depositing Organization
    Imperial College London
    Located in United Kingdom of Great Britain and Northern Ireland
  • Publication
    Ellis genetic toolkit (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235414 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSB1A2
  • Backbone manufacturer
    iGEM
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bkdB Cold double terminator
  • Species
    Other
  • Mutation
    No

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STK080_pSTK-0D-bkdB Cold double terminator was a gift from Tom Ellis (Addgene plasmid # 235414 ; http://n2t.net/addgene:235414 ; RRID:Addgene_235414)
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