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STK188_pGeoEXP-sfGFP-1
(Plasmid #235443)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235443 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pS797
  • Vector type
    Bacterial Expression
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP Dropout Cassette
  • Species
    Other
  • Mutation
    No

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STK188_pGeoEXP-sfGFP-1 was a gift from Tom Ellis (Addgene plasmid # 235443 ; http://n2t.net/addgene:235443 ; RRID:Addgene_235443)
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