pEtv5-Bsd-T2a-Fkbp-3xF-GGSG
(Plasmid #235525)

• Purpose: Etv5 N-term targeting vector, Long Isoform, Blasto-T2A
• Depositing Lab: Brian Hendrich
• Depositing Organization: University of Cambridge
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Hendrich lab plasmids batch2 (unpublished)

Backbone

• Vector backbone: pBluescript II KS-
• Backbone size w/o insert (bp): 3000
• Vector type: Mouse Targeting
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Etv5
• Species: M. musculus (mouse)
• Mutation: None
• Entrez Gene: Etv5 (a.k.a. 1110005E01Rik, 8430401F14Rik, ERM)
• Tag / Fusion Protein:
  • Bsdd, FKBP, 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry