pMO14
(Plasmid #235736)

• Purpose: Expression of human VPS34 complex II in mammalian cells
• Depositing Lab: Roger Williams
• Depositing Organization: MRC Laboratory of Molecular Biology
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi: 10.15252/embj.2020105985. Epub 2021 Jun 14.

Backbone

• Vector backbone: pcDNA4.0/TO
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Depositing lab also recommends XL10-gold E. coli for transformation.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: UVRAG
• Species: H. sapiens (human)
• Entrez Gene: UVRAG (a.k.a. DHTX, VPS38, p63)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2xStrep-tagII-2xTEV-ZZ (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: EGFP
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: BECN1
• Species: H. sapiens (human)
• Entrez Gene: BECN1 (a.k.a. ATG6, VPS30, beclin1)
• Promoter: CMV

Cloning Information for Gene/Insert 3

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: PIK3C3
• Species: H. sapiens (human)
• Entrez Gene: PIK3C3 (a.k.a. VPS34, Vps34, hVps34)
• Promoter: CMV

Cloning Information for Gene/Insert 4

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Gene/Insert 5

• Gene/Insert name: PIK3R4
• Species: H. sapiens (human)
• Entrez Gene: PIK3R4 (a.k.a. VPS15, p150)
• Promoter: CMV

Cloning Information for Gene/Insert 5

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 235736-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 235736-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.