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pYO555
(Plasmid #235745)

  • Purpose
    Protein expression of KmKog1 in yeast
  • Depositing Lab
  • Depositing Organization
    MRC Laboratory of Molecular Biology
    Located in United Kingdom of Great Britain and Northern Ireland
  • Sequence Information
    • Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235745 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pRS426
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KmKOG1
  • Species
    K. marxianus
  • Tag / Fusion Protein
    • ZZ-3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO555 was a gift from Roger Williams (Addgene plasmid # 235745 ; http://n2t.net/addgene:235745 ; RRID:Addgene_235745)