3xFLAG-TurboID-LRRK1
(Plasmid
#236118)
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PurposeExpresses a 3xFLAG-TurboID-LRRK1 fusion construct that can be used for proximity labeling in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 12189
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLRRK1
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SpeciesH. sapiens (human)
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Entrez GeneLRRK1 (a.k.a. OSMD, RIPK6, Roco1)
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Tags
/ Fusion Proteins
- 3xFLAG (N terminal on insert)
- TurboID (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3xFLAG-TurboID-LRRK1 was a gift from Samara Reck-Peterson (Addgene plasmid # 236118 ; http://n2t.net/addgene:236118 ; RRID:Addgene_236118) -
For your References section:
Probing the proteome-wide impact of inhibitors of Leucine-rich Repeat Kinases 1 and 2 on protein-protein interactions and phosphorylation. Jansen J, Wendel M, Raig ND, Kraus T, Surridge KJ, Mahesula S, Richter-Müller N, Mathea S, Reck-Peterson SL, Knapp S, Stengel F. bioRxiv 2025.09.03.674114 10.1101/2025.09.03.674114