pEB0_9_8xCusR_BSsENO1cp
(Plasmid
#236214)
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PurposePlasmid encoding 8xCusR_BSsENO1cp (eight repeats of the binding sequence of cusR directly upstream of the ENO1 core promoter) as a Type 2 part to be used in the Dueber Yeast MoClo Toolkit.
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Depositing Lab
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Depositing OrganizationChalmers University of Technology
Located in Sweden -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepEX-K248
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameENO1 core promoter with 8x binding sites for CusR
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid made are based on the Yeast MoClo Toolkit.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEB0_9_8xCusR_BSsENO1cp was a gift from Yvonne Nygård (Addgene plasmid # 236214 ; http://n2t.net/addgene:236214 ; RRID:Addgene_236214) -
For your References section:
Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions. Blick E, Nygard Y. Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf018. doi: 10.1093/synbio/ysaf018. eCollection 2025. 10.1093/synbio/ysaf018 PubMed 41439119
Map uploaded by the depositor.
