pEB2_4_CusR-VIII
(Plasmid
#236219)
-
PurposeCusR-based expression system: Plasmid with cusR-mTurquoise2-VP64-SV40 under RET2p and mCherry under 8xCusR_BSsENO1cp with homologous regions for genome integration into HO locus.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backboneMoClo YTK
-
Vector typeBacterial Expression, Synthetic Biology
-
Selectable markersURA3
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert 1
-
Gene/Insert namecusR
-
Alt namecusR-mTurquoise2-VP64-NLS, ENO1 terminator
-
SpeciesSynthetic
- Promoter RET2p
-
Tag
/ Fusion Protein
- mTurquoise2-VP64-NLS (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Golden Gate
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert namemCherry, SSA1 terminator
-
SpeciesSynthetic
- Promoter 8xCusR_BSsENO1cp
Cloning Information for Gene/Insert 2
- Cloning method Golden Gate
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid made are based on the Yeast MoClo Toolkit.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEB2_4_CusR-VIII was a gift from Yvonne Nygård (Addgene plasmid # 236219 ; http://n2t.net/addgene:236219 ; RRID:Addgene_236219) -
For your References section:
Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions. Blick E, Nygard Y. Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf018. doi: 10.1093/synbio/ysaf018. eCollection 2025. 10.1093/synbio/ysaf018 PubMed 41439119
Map uploaded by the depositor.
