p3HM1Luc4
(Plasmid #236277)

• Purpose: Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8
• Depositing Lab: Vsevolod Gurevich
• Depositing Organization: Vanderbilt University
  Located in United States of America
• Publication: Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-505. doi: 10.1074/jbc.M112.366674. Epub 2012 Jul 11.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Modifications to backbone: The MCS of pcDNA3 vector was modified
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: muscarinic M1 receptor
• Alt name: clone p3HM1Luc4
• Species: H. sapiens (human)
• Entrez Gene: CHRM1 (a.k.a. HM1, M1, M1R)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xHA (N terminal on insert)
  • Rluc8 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Eco RI (unknown if destroyed)
• 3′ cloning site: Hind III (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MCS of the pcDNA3 vector was modified, so the structure of insert is: EcoRI - AsiSI - Start 3xHA-D2R - SbfI - RLuc8 - Stop AscI - XbaI - PacI - HindIII

DNA (Catalog # 236277-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 236277-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.