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phRLucN2-Rgs14
(Plasmid #236397)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236397 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    phRLucN2
  • Backbone manufacturer
    Dr. Michel Bouvier (University of Montreal)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rattus norvegicus regulator of G-protein signaling 14
  • Alt name
    Rgs14
  • Species
    R. norvegicus (rat)
  • GenBank ID
    U92279.1
  • Entrez Gene
    Rgs14
  • Tag / Fusion Protein
    • Renilla luciferase (Luc) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer Rgs14 forward primer 5′-GCT CTC GAG GCC ACC ATG CCA GGG AAG CCC AAG CAC-3'
  • 3′ sequencing primer Rgs14 reverse primer 5′-CGC GGT ACC TGG TGG AGC CTC CTG AGA ACC-3′
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    D.P. Siderovski (REF: B E Snow, L Antonio, S Suggs, H B Gutstein, D P Siderovski (1997) Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun. Apr 28;233(3):770-7. doi: 10.1006/bbrc.1997.6537. PMID: 9168931)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phRLucN2-Rgs14 was a gift from John Hepler (Addgene plasmid # 236397 ; http://n2t.net/addgene:236397 ; RRID:Addgene_236397)

  • For your References section:

    G protein-coupled receptors and resistance to inhibitors of cholinesterase-8A (Ric-8A) both regulate the regulator of g protein signaling 14 RGS14.Galphai1 complex in live cells. Vellano CP, Maher EM, Hepler JR, Blumer JB. J Biol Chem. 2011 Nov 4;286(44):38659-38669. doi: 10.1074/jbc.M111.274928. Epub 2011 Aug 31. 10.1074/jbc.M111.274928 PubMed 21880739
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