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His-SUMO-Rab10 Q68L
(Plasmid #236720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236720 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15b+
  • Backbone size w/o insert (bp) 5654
  • Total vector size (bp) 6563
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab10 Q68L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    912
  • Mutation
    Q68L
  • Entrez Gene
    RAB10
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-SUMO (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.19.644182 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-SUMO-Rab10 Q68L was a gift from Suzanne Pfeffer (Addgene plasmid # 236720 ; http://n2t.net/addgene:236720 ; RRID:Addgene_236720)

  • For your References section:

    PPM1M, an LRRK2-counteracting, phosphoRab12-preferring phosphatase with a potential link to Parkinson's disease. Chiang CY, Pratuseviciute N, Lin YE, Adhikari A, Yeshaw WM, Flitton C, Sherpa PL, Tonelli F, Rektorova I, Lynch T, Siuda J, Rudzinska-Bar M, Pulyk O, Bauer P, Beetz C, Dickson DW, Ross OA, Wszolek ZK, Fang ZH, Klein C, Zimprich A, Alessi DR, Sammler EM, Pfeffer SR. Cell Rep. 2025 Aug 26;44(8):116031. doi: 10.1016/j.celrep.2025.116031. Epub 2025 Jul 20. 10.1016/j.celrep.2025.116031 PubMed 40690364
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