HDM_RSV_B_OQ279862_F_N201T
(Plasmid
#237362)
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PurposeExpression plasmid for RSV B F from OQ279862
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Depositing Lab
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Depositing OrganizationFred Hutchinson Cancer Center
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 237362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 237362-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 237362-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneHDM
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRSV B F human codon optimized from Genbank Accesion OQ27986
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SpeciesRSV
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Clinical sequence selected for presence of N201T in RSV F, a known nirsevimab escape mutant.
Please visit https://doi.org/10.1101/2025.03.11.642476 for bioRxiv preprint.
DNA (Catalog # 237362-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 237362-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HDM_RSV_B_OQ279862_F_N201T was a gift from Jesse Bloom (Addgene plasmid # 237362 ; http://n2t.net/addgene:237362 ; RRID:Addgene_237362) -
For your References section:
RSV F evolution escapes some monoclonal antibodies but does not strongly erode neutralization by human polyclonal sera. Simonich CAL, McMahon TE, Ju X, Yu TC, Brunette N, Stevens-Ayers T, Boeckh MJ, King NP, Greninger AL, Bloom JD. J Virol. 2025 Jul 3:e0053125. doi: 10.1128/jvi.00531-25. 10.1128/jvi.00531-25 PubMed 40607811
