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Vtc2p_SPX_KSC
(Plasmid #237894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 237894 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMH (pET24d-based)
  • Backbone size w/o insert (bp) 5206
  • Total vector size (bp) 5779
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vtc2p_SPX_1-182
  • Alt name
    vtc2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    546
  • Mutation
    K127A, K130A, K134A
  • Entrez Gene
    VTC2 (a.k.a. YFL004W, PHM1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rebekka Wild, doi: 10.1126/science.aad9858

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vtc2p_SPX_KSC was a gift from Michael Hothorn (Addgene plasmid # 237894 ; http://n2t.net/addgene:237894 ; RRID:Addgene_237894)

  • For your References section:

    The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions. Sturm K, Hothorn M. Methods Mol Biol. 2025;2972:153-169. doi: 10.1007/978-1-0716-4799-8_12. 10.1007/978-1-0716-4799-8_12 PubMed 40879985
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